Two-Way Antigenic Cross-Reactivity between Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Group 1 Animal CoVs Is Mediated through an Antigenic Site in the N-Terminal Region of the SARS-CoV Nudeoprotein
Identifieur interne : 003B11 ( Main/Exploration ); précédent : 003B10; suivant : 003B12Two-Way Antigenic Cross-Reactivity between Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Group 1 Animal CoVs Is Mediated through an Antigenic Site in the N-Terminal Region of the SARS-CoV Nudeoprotein
Auteurs : Anastasia N. Vlasova [États-Unis] ; XINSHENG ZHANG [États-Unis] ; Mustafa Hasoksuz [Turquie] ; Hadya S. Nagesha [États-Unis] ; Lia M. Haynes [États-Unis] ; YING FANG [États-Unis] ; SHAN LU [États-Unis] ; Linda J. Saif [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Antigènes viraux (immunologie), Antigènes viraux (sang), Bovins, Chats, Chiens, Cochons d'Inde, Coronavirus (), Coronavirus (immunologie), Humains, Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Lignée cellulaire, Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Réactions croisées, Syndrome respiratoire aigu sévère (virologie), Sérums immuns (immunologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Protéines nucléocapside.
- immunologie : Anticorps antiviraux, Antigènes viraux, Coronavirus, Protéines nucléocapside, Sérums immuns, Virus du SRAS.
- médecine vétérinaire : Infections à coronavirus.
- sang : Anticorps antiviraux, Antigènes viraux.
- virologie : Infections à coronavirus, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antigens, Viral (blood), Antigens, Viral (immunology), Cats, Cattle, Cell Line, Coronavirus (classification), Coronavirus (immunology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Cross Reactions, Cross reaction, Dogs, Guinea Pigs, Humans, Immune Sera (immunology), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.
- MESH :
- chemical , blood : Antibodies, Viral, Antigens, Viral.
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Immune Sera, Nucleocapsid Proteins.
- classification : Coronavirus.
- immunology : Coronavirus, SARS Virus.
- veterinary : Coronavirus Infections.
- virology : Coronavirus Infections, Severe Acute Respiratory Syndrome.
- Animals, Cats, Cattle, Cell Line, Cross Reactions, Dogs, Guinea Pigs, Humans.
Abstract
In 2002, severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged in humans, causing a global epidemic. By phylogenetic analysis, SARS-CoV is distinct from known CoVs and most closely related to group 2 CoVs. However, no antigenic cross-reactivity between SARS-CoV and known CoVs was conclusively and consistently demonstrated except for group 1 animal CoVs. We analyzed this cross-reactivity by an enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using specific antisera to animal CoVs and SARS-CoV and SARS patient convalescent-phase or negative sera. Moderate two-way cross-reactivity between SARS-CoV and porcine CoVs (transmissible gastroenteritis CoV [TGEV] and porcine respiratory CoV [PRCV]) was mediated through the N but not the spike protein, whereas weaker cross-reactivity occurred with feline (feline infectious peritonitis virus) and canine CoVs. Using Escherichia coli-expressed recombinant SARS-CoV N protein and fragments, the cross-reactive region was localized between amino acids (aa) 120 to 208. The N-protein fragments comprising aa 360 to 412 and aa 1 to 213 reacted specifically with SARS convalescent-phase sera but not with negative human sera in ELISA; the fragment comprising aa 1 to 213 cross-reacted with antisera to animal CoVs, whereas the fragment comprising aa 360 to 412 did not cross-react and could be a potential candidate for SARS diagnosis. Particularly noteworthy, a single substitution at aa 120 of PRCV N protein diminished the cross-reactivity. We also demonstrated that the cross-reactivity is not universal for all group 1 CoVs, because HCoV-NL63 did not cross-react with SARS-CoV. One-way cross-reactivity of HCoV-NL63 with group 1 CoVs was localized to aa 1 to 39 and at least one other antigenic site in the N-protein C terminus, differing from the cross-reactive region identified in SARS-CoV N protein. The observed cross-reactivity is not a consequence of a higher level of amino acid identity between SARS-CoV and porcine CoV nucleoproteins, because sequence comparisons indicated that SARS-CoV N protein has amino acid identity similar to that of infectious bronchitis virus N protein and shares a higher level of identity with bovine CoV N protein within the cross-reactive region. The TGEV and SARS-CoV N proteins are RNA chaperons with long disordered regions. We speculate that during natural infection, antibodies target similar short antigenic sites within the N proteins of SARS-CoV and porcine group 1 CoVs that are exposed to an immune response. Identification of the cross-reactive and non-cross-reactive N-protein regions allows development of SARS-CoV-specific antibody assays for screening animal and human sera.
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Affiliations:
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Le document en format XML
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<author><name sortKey="Ying Fang" sort="Ying Fang" uniqKey="Ying Fang" last="Ying Fang">YING FANG</name>
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<author><name sortKey="Shan Lu" sort="Shan Lu" uniqKey="Shan Lu" last="Shan Lu">SHAN LU</name>
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<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antigens, Viral (blood)</term>
<term>Antigens, Viral (immunology)</term>
<term>Cats</term>
<term>Cattle</term>
<term>Cell Line</term>
<term>Coronavirus (classification)</term>
<term>Coronavirus (immunology)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Coronavirus Infections (virology)</term>
<term>Cross Reactions</term>
<term>Cross reaction</term>
<term>Dogs</term>
<term>Guinea Pigs</term>
<term>Humans</term>
<term>Immune Sera (immunology)</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
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<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Antigènes viraux (immunologie)</term>
<term>Antigènes viraux (sang)</term>
<term>Bovins</term>
<term>Chats</term>
<term>Chiens</term>
<term>Cochons d'Inde</term>
<term>Coronavirus ()</term>
<term>Coronavirus (immunologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Lignée cellulaire</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Réactions croisées</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Sérums immuns (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Antigens, Viral</term>
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</keywords>
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<term>Antigens, Viral</term>
<term>Immune Sera</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term>
</keywords>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Coronavirus</term>
<term>Protéines nucléocapside</term>
<term>Sérums immuns</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term>
<term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cats</term>
<term>Cattle</term>
<term>Cell Line</term>
<term>Cross Reactions</term>
<term>Dogs</term>
<term>Guinea Pigs</term>
<term>Humans</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Bovins</term>
<term>Chats</term>
<term>Chiens</term>
<term>Cochons d'Inde</term>
<term>Coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéines nucléocapside</term>
<term>Réactions croisées</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Réaction croisée</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In 2002, severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged in humans, causing a global epidemic. By phylogenetic analysis, SARS-CoV is distinct from known CoVs and most closely related to group 2 CoVs. However, no antigenic cross-reactivity between SARS-CoV and known CoVs was conclusively and consistently demonstrated except for group 1 animal CoVs. We analyzed this cross-reactivity by an enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using specific antisera to animal CoVs and SARS-CoV and SARS patient convalescent-phase or negative sera. Moderate two-way cross-reactivity between SARS-CoV and porcine CoVs (transmissible gastroenteritis CoV [TGEV] and porcine respiratory CoV [PRCV]) was mediated through the N but not the spike protein, whereas weaker cross-reactivity occurred with feline (feline infectious peritonitis virus) and canine CoVs. Using Escherichia coli-expressed recombinant SARS-CoV N protein and fragments, the cross-reactive region was localized between amino acids (aa) 120 to 208. The N-protein fragments comprising aa 360 to 412 and aa 1 to 213 reacted specifically with SARS convalescent-phase sera but not with negative human sera in ELISA; the fragment comprising aa 1 to 213 cross-reacted with antisera to animal CoVs, whereas the fragment comprising aa 360 to 412 did not cross-react and could be a potential candidate for SARS diagnosis. Particularly noteworthy, a single substitution at aa 120 of PRCV N protein diminished the cross-reactivity. We also demonstrated that the cross-reactivity is not universal for all group 1 CoVs, because HCoV-NL63 did not cross-react with SARS-CoV. One-way cross-reactivity of HCoV-NL63 with group 1 CoVs was localized to aa 1 to 39 and at least one other antigenic site in the N-protein C terminus, differing from the cross-reactive region identified in SARS-CoV N protein. The observed cross-reactivity is not a consequence of a higher level of amino acid identity between SARS-CoV and porcine CoV nucleoproteins, because sequence comparisons indicated that SARS-CoV N protein has amino acid identity similar to that of infectious bronchitis virus N protein and shares a higher level of identity with bovine CoV N protein within the cross-reactive region. The TGEV and SARS-CoV N proteins are RNA chaperons with long disordered regions. We speculate that during natural infection, antibodies target similar short antigenic sites within the N proteins of SARS-CoV and porcine group 1 CoVs that are exposed to an immune response. Identification of the cross-reactive and non-cross-reactive N-protein regions allows development of SARS-CoV-specific antibody assays for screening animal and human sera.</div>
</front>
</TEI>
<affiliations><list><country><li>Turquie</li>
<li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
</region>
<settlement><li>Amherst (Massachusetts)</li>
</settlement>
<orgName><li>Université du Massachusetts</li>
</orgName>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Vlasova, Anastasia N" sort="Vlasova, Anastasia N" uniqKey="Vlasova A" first="Anastasia N." last="Vlasova">Anastasia N. Vlasova</name>
</noRegion>
<name sortKey="Haynes, Lia M" sort="Haynes, Lia M" uniqKey="Haynes L" first="Lia M." last="Haynes">Lia M. Haynes</name>
<name sortKey="Nagesha, Hadya S" sort="Nagesha, Hadya S" uniqKey="Nagesha H" first="Hadya S." last="Nagesha">Hadya S. Nagesha</name>
<name sortKey="Saif, Linda J" sort="Saif, Linda J" uniqKey="Saif L" first="Linda J." last="Saif">Linda J. Saif</name>
<name sortKey="Shan Lu" sort="Shan Lu" uniqKey="Shan Lu" last="Shan Lu">SHAN LU</name>
<name sortKey="Xinsheng Zhang" sort="Xinsheng Zhang" uniqKey="Xinsheng Zhang" last="Xinsheng Zhang">XINSHENG ZHANG</name>
<name sortKey="Ying Fang" sort="Ying Fang" uniqKey="Ying Fang" last="Ying Fang">YING FANG</name>
</country>
<country name="Turquie"><noRegion><name sortKey="Hasoksuz, Mustafa" sort="Hasoksuz, Mustafa" uniqKey="Hasoksuz M" first="Mustafa" last="Hasoksuz">Mustafa Hasoksuz</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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